Vol. 4, Issue 3 (2019)
Optimization of the enzyme-assisted vacuum-distillation of essential oil from the leaves of Citrus limonia Osbeck by response surface method
Author(s): Pham Thi Lan Chi, Le Huu Thanh, Nguyen Thi Lan Phi
Abstract: The process of enzyme-assisted vacuum-distillation for extraction of leaves essential oil from Citrus limonia Osbeck was studied and optimized using response surface methodology (RSM). The factors considered were material to water ratio (A), incubation time (B) and concentration of Viscozyme-L (C). These parameters were varied at two levels. The material to water ratio of 0.26 g/ml, incubation time 71.25 min and Viscozyme -L concentration of 8.46 U/ml were predicted as the optimum conditions. These factors gave an optimum oil yield of 0.28%. Analysis of variance (ANOVA) indicates that the model was significant as evidenced from R2 of 0.9018 and the model F-value of 6.12. The experimental value (0.27 % oil yield) was close to the predicted value (0.28%). Therefore, the model can be used for prediction of oil yield in essential oil extraction from Citrus limonia Osbeck leaves via enzyme-assisted vacuum-distillation method.