Vol. 3, Issue 4 (2018)
In vitro diseases cleaning, micro-propagation and field establishment of ginger (Zingiber officinale Rosc.) cultivars
Author(s): Berihu Mengs
Abstract: Ginger (Zingiber officinale Rosc.) is an important tropical and sub-tropical herbaceous perennial plant widely cultivated spice in Ethiopia. Recently, there has been huge demand for clean planting material of improved ginger cultivars, though it is difficult to meet the demand of planting materials using the conventional propagation techniques due to production inefficiency and disease infected rhizome seeds. The morphologically good and healthy sprout buds were washed under running tap water supplemented with detergents followed by surface sterilization in 3%(w/v) CuSo4 supplemented with three drops of Tween 20 for 15 min and rinsed with sterile water and the explants were treated by 70% (v/v) ethanol for 1min in laminar air flow hood, flashed again in sterilized water. Then, we were disinfected using 30% (v/v) sodium hypochlorite (5% active chlorine ingredient) for 15 min under laminar air flow hood. Therefore, in vitro disease cleaning and mass propagation of ginger were examined by biochemical and serological test via NCM-ELISA. The costumer ID of G04, G06, G10, G15 and G17 were not showed (negative) results of ginger sample raised in in vitro Initiation, multiplication, rooting, Acclimatization and rhizome setting respectively. In addition to that, disease free ginger plantlets were mass propagated and commercialized to our customers.